Table 1.
Validation parameters for phenolics analysis by UPLC-HRMS-Orbitrap.
| Phenolic Compounds | Linearity (mg/L) |
R2 | LOD (mg/L) |
LOQ (mg/L) |
Intraday RSD % (n = 3), 50 mg/L |
|---|---|---|---|---|---|
| Phenolic Acids | |||||
| 4-Hydroxybenzoic acid | 1–50 | 0.998 | 0.207 | 0.622 | 0.9 |
| 3-Hydroxybenzoic acid | 1–50 | 0.995 | 0.205 | 0.622 | 1.1 |
| Vanillic acid | 1–50 | 0.887 | 0.200 | 0.600 | 1.1 |
| Cinnamic acid | 1–50 | 0.991 | 0.200 | 0.600 | 0.9 |
| p-Coumaric acid | 1–50 | 1.000 | 0.100 | 0.300 | 1.8 |
| Ferulic acid | 1–50 | 0.912 | 0.100 | 0.300 | 1.7 |
| Flavonoids | |||||
| Luteolin | 0.5–50 | 0.991 | 0.066 | 0.200 | 1.4 |
| Apigenin | 0.5–50 | 0.899 | 0.066 | 0.200 | 2.1 |
| Lignans | |||||
| (+)Pinoresinol | 1–50 | 0.999 | 0.02 | 0.060 | 0.5 |
| (+)1-Acetoxypinoresinol | 1–50 | 0.899 | 0.233 | 0.700 | 1.5 |
| Phenolic Alcohols | |||||
| p-HPEA (Tyrosol) | 1–50 | 0.991 | 0.133 | 0.040 | 1.6 |
| 3.4 DHPEA (Hydroxytyrosol) | 1–50 | 0.992 | 0.666 | 2.000 | 3.0 |
| Secoiridoids | |||||
| Oleuropein | 1–50 | 0.991 | 0.166 | 0.500 | 5.0 |
| Ligstroside | 1–50 | 0.991 | 0.166 | 0.500 | 4.0 |
| Secologanoside | 1–50 | 0.967 | 0.333 | 1.000 | 2.1 |
| Elenaic acid | 1–50 | 0.991 | 0.333 | 1.000 | 0.7 |
| 3.4 DHPEA-EDA (Oleuropein aglycone dialdehyde) 3.4-DHPEA-EA (Oleuropein-aglycone monoaldehyde) |
1–50 | 0.998 | 1.000 | 3.000 | 2.1 |
| p-HPEA-EDA (Ligstroside-aglycone dialdehyde) | 1–50 | 0.899 | 0.416 | 1.250 | 3.0 |