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. 2020 Mar 25;9(4):275. doi: 10.3390/antiox9040275

Figure 5.

Figure 5

Corylin reduces the activation of NF-κB p65 in TNF-α-treated HUVECs and VSMCs. (A) HUVECs were pretreated (1 h) with 20 µM corylin (C20) or 30 nM SP600125 (SP30) and then incubated with 10 ng/mL TNF-α (T) for 15 min. VSMCs were pretreated (1 h) with 20 µM corylin, 30 µM SB203580 (SB30), 30 µM PD98059 (PD30), or 30 nM SP600125 (SP30) and then incubated with 10 ng/mL TNF-α (T) for 15 min. Immunofluorescence staining was performed to examine the nuclear localization and expression level of phosphorylated NF-κB p65 (P-p65). Nuclei were labeled with DAPI (blue). Scale bar = 25 μm. (B,C) HUVECs and VSMCs were transfected with NF-κB p65 luciferase reporter constructs, pretreated (1 h) with 20 µM corylin (C20), 30 µM SB203580 (SB30), 30 µM PD98059 (PD30), or 30 nM SP600125 (SP30) and then incubated with 10 ng/mL TNF-α for 6 h. Total cell lysates were collected, and luciferase activity was detected. The data are shown as the mean ± SD. * p <0.05 versus the untreated group (CTRL). † p < 0.05 versus the TNF-α-treated group.