Figure 6.

Betaine treatment enhanced OGD/R treated primary neuronal death and CL treatment attenuated OGD/R-treated primary neuronal death. (A–C) Primary neurons were treated with 8 mM Betaine or 20mM CL and then cultured under OGD/R conditions or normoxia. Cell death was quantified by CCK8 assay or by microscopy for PI-positive cells. Quantifications of the percentage of dead cells were shown. (mean ± SEM; n = 6–20; *p < 0.05, ***p < 0.001 versus OGD/R). (D–E) Representative images of Western blots of primary neuron treated with 8 mM Betaine or 20 mM CL. Cleaved-caspase3 levels were determined by Western blot (mean ± SEM; n = 3; *p < 0.05 versus OGD/R). (F) A proposed model for Alkbh5 and Fto in cerebral ischemia-reperfusion injury.

CCK8, Cell Counting Kit-8; CL, cycloleucine; OGD/R, glucose oxygen deprivation/reoxygenation; PI, propidium iodide; SEM, standard error of the mean.