Table 4.
Results for the statistical analysis of the relative mRNA expression of the selected antigens in the cell lines examined.
| Gene | Cell comparison | Significance | p-value |
|---|---|---|---|
| TRIM28 | U87MG versus NCH | **** | <0.0001 |
| U87MG versus U251 | **** | <0.0001 | |
| U87MG versus astrocytes | **** | <0.0001 | |
| NUCL | U87MG versus NCH | *** | 0.0003 |
| U87MG versus U251 | **** | <0.0001 | |
| Astrocytes versus U251 | ** | 0.0083 | |
| VIM | U251MG versus NCH | **** | <0.0001 |
| U87MG versus NCH | **** | <0.0001 | |
| U251MG versus U87MG | **** | <0.0001 | |
| U87MG versus astrocytes | **** | <0.0001 | |
| U251MG versus astrocytes | **** | <0.0001 | |
| NAP1L1 | NCH versus U251MG | **** | <0.0001 |
| NCH versus U87MG | *** | 0.0008 | |
| NCH versus astrocytes | **** | <0.0001 | |
| U87MG versus U251 | **** | <0.0001 | |
| U87MG versus astrocytes | **** | <0.0001 | |
| TUFM | NCH versus U251MG | **** | <0.0001 |
| NCH versus U87MG | **** | <0.0001 | |
| NCH versus astrocytes | **** | <0.0001 | |
| U251MG versus U87MG | ** | 0.0013 | |
| DPYSL2 | U87MG versus U251 | *** | 0.0001 |
| NCH versus U251MG | ** | 0.0077 | |
| CRMP1 | NCH versus U251MG | **** | <0.0001 |
| NCH versus astrocytes | **** | <0.0001 | |
| ALYREF | U87MG versus NCH | ** | 0.0056 |
| U87MG versus U251 | ** | 0.0075 | |
| U87MG versus astrocytes | *** | 0.0002 |
Significance for nine replicates for each gene that followed a Gaussian distribution was calculated using one-way ANOVA, and for the values without a Gaussian distribution, by Kruskal-Wallis statistical tests.
**p < 0.01, ***p < 0.001, ****p < 0.0001.
ALYREF, Aly/REF export factor; CRMP1, collapsin response mediator protein 1; DPYSL2, dihydropyrimidinase-related protein 2; NAP1L1, nucleosome assembly protein 1-like 1; NUCL, nucleolin; RPL13A, ribosomal protein L13a; TRIM28, tripartite motif containing 28 protein; TUFM, mitochondrial translation elongation factor (EF-TU); VIM, vimentin.