Table 5.
Statistical analysis of protein expression for the selected antigens in the different cell lines.
| Protein | Cell comparison | Significance | p-value |
|---|---|---|---|
| TRIM28 | All | ns | |
| NUCL | All | ns | |
| VIM | U87MG versus NCH | * | 0.0130 |
| NCH versus astrocytes | * | 0.0263 | |
| U87MG versus U251 | ** | 0.0053 | |
| U87MG versus astrocytes | *** | 0.0002 | |
| NAP1L1 | NCH versus U251MG | *** | 0.0004 |
| NCH versus U87MG | * | 0.0286 | |
| NCH versus astrocytes | *** | 0.0009 | |
| TUFM | All | ns | |
| DPYSL2 | NCH versus U251MG | **** | <0.0001 |
| NCH versus U87MG | **** | <0.0001 | |
| NCH versus astrocytes | **** | <0.0001 | |
| CRMP1 | NCH versus U251MG | ** | 0.0015 |
| NCH versus U87MG | ** | 0.0044 | |
| NCH versus astrocytes | ** | 0.0060 | |
| ALYREF | NCH versus U251MG | ** | 0.0093 |
| NCH versus U87MG | * | 0.0108 | |
| NCH versus astrocytes | ** | 0.0043 |
Significance of three samples was calculated using Student’s t-tests. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, non-significant.
ALYREF, Aly/REF export factor; CRMP1, collapsin response mediator protein 1; DPYSL2, dihydropyrimidinase-related protein 2; NAP1L1, nucleosome assembly protein 1-like 1; NUCL, nucleolin; TRIM28, tripartite motif containing 28 protein; TUFM, mitochondrial translation elongation factor (EF-TU); VIM, vimentin.