Table 6.
Statistical analysis from the immunohistochemical detection of the selected proteins in normal brain, and in GB and LGG tissue.
| Antigen | Expression in normal brain tissue |
Positive tissues (%) |
Score |
Statistical analysis (GB versus LGG) |
|||
|---|---|---|---|---|---|---|---|
| GB | LGG | GB | LGG | Significance | p-value | ||
| TRIM28 | Yes | 100 | 100 | 3.8 | 3.875 | No | |
| NUCL | Yes | 100 | 100 | 3.91 | 4 | No | |
| VIM | No † | 100 | 100 | 3.91 | 1.5 | Yes | 4.3 × 10–6 |
| NAP1L1 | No | 75 | 62.5 | 1.42 | 0.875 | No | |
| TUFM | No | 100 | 100 | 2.3 | 1.875 | No | |
| DPYSL2 | Yes‡ | 100 | 100 | 2.33 | 1.875 | No | |
| CRMP1 | Yes‡ | 100 | 100 | 1.64 | 2.125 | No | |
| ALYREF | Yes | 100 | 100 | 4 | 3.875 | No | |
Significance of samples (for numbers see Table 3) was calculated using Student’s t tests.
†
VIM expression in glia cells and neurons was negative, but positive in blood vessels.
‡
DPYSL2 and CRMP1 expression was positive only in neuropils.
ALYREF, Aly/REF export factor; CRMP1, collapsin response mediator protein 1; DPYSL2, dihydropyrimidinase-related protein 2; GB, glioblastoma; LGG, lower-grade glioma; NAP1L1, nucleosome assembly protein 1-like 1; NUCL, nucleolin; TRIM28, tripartite motif containing 28 protein; TUFM, mitochondrial translation elongation factor (EF-TU); VIM, vimentin.