Table 7.

Expression of the antigens in the different cell lines and tissues according to each of the methods used in this study [i.e. qPCR, ELISA, (ICC); tissue].

Antigen	Cell lines												Tissue
	U251MG cells			U87MG cells			NCH co-culture			Astrocytes			IHC
	qPCR	ELISA	ICC	qPCR	ELISA	ICC	qPCR	ELISA	ICC	qPCR	ELISA	ICC	Normal	GB	LGG
TRIM28	+	+	+	+	+	+	+	+	+	+	+	–	+	+	+
Nucleolin	+	+	+	+	+	+	+	+	+	+	+	–	+	+	+
Vimentin	+	+	+	+	+	+	+	+	+	+	+	–	–	+	+
NAP1L1	+	+	+	+	+	+	+	+	+	+	+	–	–	+/–	+/–
TUFM	+	+	+	+	+	+	+	+	+	+	+	+	–	+	+
DPYSL2	+	+	+	+	+	+	+	+	+	+	+	+	–	+	+
CRMP1	+	+	+	+	+	+	+	+	+	+	+	–	–	+	+
ALYREF	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+

Data are presented as either positive (+) or negative (–) for expression.

NAP1L1 showed positive expression in some tissues and negative expression in others (+/–).

ALYREF, Aly/REF export factor; CRMP1, collapsin response mediator protein 1; DPYSL2, dihydropyrimidinase-related protein 2; ELISA, enzyme-linked immunosorbent assay; GB, glioblastoma; ICC, immunocytochemistry; IHC, immunohistochemistry; LGG, lower-grade glioma; NAP1L1, nucleosome assembly protein 1-like 1; Normal, normal human brain; NUCL, nucleolin; qPCR, quantitative polymerase chain reaction; TRIM28, tripartite motif containing 28 protein; TUFM, mitochondrial translation elongation factor (EF-TU); VIM, vimentin.