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. 2020 May 13;51:67. doi: 10.1186/s13567-020-00792-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Course of IL4I1 mRNA expression in the chicken cecum afterS. Enteritidis infection determined by real-time PCR. Chickens were infected at the day of hatch, sacrificed at time points as indicated and expression of IL4I1 was determined by reverse transcribed real time PCR. On days 3, 6, 9, 10 and 29, absolute Ct values for IL4I1 were below a value 30 in 2 out 4 tested non-infected chickens, and on days 11 and 12 absolute Ct values for IL4I1 were below 30 in 1 out 4 non-infected chickens indicating low IL4I expression in the cecum in the absence of infection. Asterisks—statistically significant differences from non-infected chickens (Mann–Whitney U test, P ≤ 0.05).