Table 2.
Universal or degenerate primers for divergent regions of 28S rDNA for the nine mite species
| Gene fragment | Length (bp) | Primers (5’-3’) | Tm (°C) |
|---|---|---|---|
| D2 | 532 | F: AACAAGTACHGTGAKGGAA | 52 |
| R: CTCCTTGGTCCGTGTTTC | |||
| D3 | 252 | F: GTCTTGAAACACGGACCAA | 54 |
| R: ATCGATTTGCACGTCAGAA | |||
| D5 | 419 | F: TTCTGACGTGCAAATCGAT | 55 |
| R: GGCAGGTGAGTTGTTACACA | |||
| D6 | 202 | F: GTGTGTAACAACTCACCTGCC | 55 |
| R: TTGCTACTACCACCAAGATCTG | |||
| D7 | 465 | F: CAGATCTTGGTGGTAGTAGCA | 55 |
| R: CCTTGGAGACCTGCTGC | |||
| D8 | 314 | F: GCAKCAGGTCTCCAAGG | 52 |
| R: GTTTTAATTAGACAGTCGGATTC |
Abbreviations: F, forward; R, reverse; Tm, melting temperature
Note: The letters in bold in primers indicate degenerate bases