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. 2020 May 13;22:113. doi: 10.1186/s13075-020-02207-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Gene expression and immunohistological analysis of fibrotic markers: alpha smooth muscle actin (α-SMA) (ACTA2), collagen type-1 (COL1A1) and procollagen-lysine, 2-oxoglutarate 5-dioxygenase (PLOD2) in synovial tissues isolated from 2- and 9-month-old gene-trap (Prg4^GT/GT) and 2- and 9-month-old recombined gene-trap (Prg4^GTR/GTR) mice. The Prg4 gene-trap allele is a loss of function allele (Prg4^GT/GT) whose function is restored with Cre-excision (Prg4^GTR/GTR). Recombination occurred at 3 weeks of age using intraperitoneal tamoxifen (0.1 mg/g in 100 μL corn oil vehicle) daily for 10 days. Fibrotic markers were probed using specific antibodies and staining intensities (lumens per square millimeter) were quantified. *p < 0.001; **p < 0.01; ***p < 0.05. Scale = 100 μm. aACTA2 expression was higher in 9-month Prg4^GT/GT compared to 2-month littermates and was reduced by Prg4 re-expression. b Representative images showing enhanced α-SMA immunostaining in 2- and 9-month Prg4^GT/GT synovia. cPrg4 re-expression reduced α-SMA content in 2- and 9-month-old animals. dCOL1A1 expression was higher in 9-month-old Prg4^GT/GT animals compared to 2-month and was reduced by Prg4 re-expression. e Representative images showing enhanced collagen type-I immunostaining in 9-month-old Prg4^GT/GT synovia. f Collagen type-I content was higher in 9-month-old Prg4^GT/GT animals and was reduced with Prg4 re-expression. gPLOD2 expression was higher in 9-month-old Prg4^GT/GT animals compared to 2-month and was reduced by Prg4 re-expression. h Representative images showing enhanced PLOD2 immunostaining in Prg4^GT/GT synovia. iPrg4 re-expression reduced PLOD2 content in Prg4^GT/GT synovia in 2- and 9-month-old animals