Figure 3.

Absence of Prdx6 altered the IGF-1/Akt-1/FOXO1 pathway inducing Sarcopenia. (a) Mice muscle strength was measured by performing grip test in wt (white bar) and Prdx6^-/- mice (black bar). (b) Sera levels of IGF-1 were evaluated. (c) Akt-1 phosphorylated (pAkt-1) levels at Ser⁴⁷³ were evaluated in gastrocnemius muscle lysate of Prdx6^-/- and wt mice. (d) Subcellular localization of FOXO1 was analyzed in skeletal muscle of wt and Prdx6^-/- mice. * p < 0.05 Prdx6^-/- versus wt mice. (e) MDA adducts were in gastrocnemius muscle lysate of Prdx6^-/- and wt mice. Data are expressed as mean ± SE. * p < 0.05 using Student t test. Graphs illustrate one of three separate studies, all yielding similar results (n = 5 mice per group). a.u. = arbitrary units; FI = fluorescence intensity; C = cytoplasm; N = nucleus.