Table 1.
Experimental runs using coded levels of time (min, x1), temperature (°C, x2) and solid: liquid ratio (g/mL, x3) according to the 23 full factorial central composite design and data of total phenolic content (TPC) and reducing antioxidant activity (FRAP) of extracts obtained under those conditions for the tested experimental model (EtOH 0%, EtOH 30%, EtOH 50%, EtOH 70% and EtOH 90%).
| Runs | Time (min) x1 |
Temperature (°C) x2 |
Solid-Liquid Ratio (g/mL) x3 |
EtOH (v/v) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0% (H2O) | 30% | 50% | 70% | 90% | |||||||||
| TPC | FRAP | TPC | FRAP | TPC | FRAP | TPC | FRAP | TPC | FRAP | ||||
| 1 | 35 (−1) | 38 (−1) | 0.05 (−1) | 18.70 | 0.13 | 20.81 | 0.29 | 68.56 | 0.42 | 60.94 | 0.44 | 59.74 | 0.34 |
| 2 | 35 (−1) | 38 (−1) | 0.15 (1) | 31.77 | 0.26 | 93.44 | 0.93 | 99.04 | 1.15 | 97.34 | 1.06 | 91.54 | 0.83 |
| 3 | 35 (−1) | 82 (1) | 0.05 (−1) | 20.18 | 0.17 | 60.94 | 0.35 | 69.04 | 0.48 | 71.04 | 0.50 | 61.74 | 0.34 |
| 4 | 35 (−1) | 82 (1) | 0.15 (1) | 37.59 | 0.33 | 106.14 | 0.99 | 119.44 | 1.39 | 126.24 | 1.15 | 98.84 | 0.97 |
| 5 | 115 (1) | 38 (−1) | 0.05 (−1) | 19.86 | 0.16 | 58.14 | 0.32 | 61.34 | 0.43 | 63.44 | 0.45 | 60.64 | 0.37 |
| 6 | 115 (1) | 38 (−1) | 0.15 (1) | 31.76 | 0.26 | 86.84 | 0.94 | 99.04 | 1.40 | 108.54 | 1.11 | 99.94 | 0.61 |
| 7 | 115 (1) | 82 (1) | 0.05 (−1) | 22.68 | 0.16 | 75.74 | 0.41 | 93.24 | 0.58 | 82.04 | 0.60 | 62.44 | 0.43 |
| 8 | 115 (1) | 82 (1) | 0.15 (1) | 48.13 | 0.35 | 120.14 | 1.35 | 163.64 | 1.50 | 136.54 | 1.44 | 123.84 | 1.14 |
| 9 | 8 (−1.682) | 60 (0) | 0.1 (0) | 5.30 | 0.08 | 72.24 | 0.49 | 77.24 | 0.61 | 76.34 | 0.55 | 75.64 | 0.53 |
| 10 | 142 (1.682) | 60 (0) | 0.1 (0) | 30.59 | 0.25 | 83.14 | 0.71 | 102.54 | 1.21 | 100.24 | 0.96 | 89.34 | 0.94 |
| 11 | 75 (0) | 23 (−1.682) | 0.1 (0) | 20.31 | 0.18 | 69.24 | 0.49 | 79.54 | 0.66 | 81.54 | 0.57 | 73.64 | 0.51 |
| 12 | 75 (0) | 97 (1.682) | 0.1 (0) | 35.61 | 0.29 | 95.14 | 1.08 | 115.94 | 1.07 | 124.34 | 1.43 | 104.24 | 0.96 |
| 13 | 75 (0) | 60 (0) | 0.016 (−1.682) | 9.08 | 0.09 | 19.38 | 0.17 | 59.69 | 0.21 | 57.64 | 0.38 | 25.35 | 0.17 |
| 14 | 75 (0) | 60 (0) | 0.184 (1.682) | 36.09 | 0.33 | 118.64 | 1.30 | 138.84 | 1.34 | 133.04 | 1.40 | 115.64 | 1.07 |
| 15 | 75 (0) | 60 (0) | 0.1 (0) | 27.97 | 0.25 | 83.64 | 1.01 | 92.54 | 1.09 | 106.94 | 0.94 | 89.14 | 0.81 |
| 16 | 75 (0) | 60 (0) | 0.1 (0) | 29.24 | 0.26 | 94.94 | 0.79 | 92.74 | 0.98 | 99.24 | 1.01 | 85.04 | 0.78 |
| 17 | 75 (0) | 60 (0) | 0.1 (0) | 28.28 | 0.25 | 91.34 | 1.00 | 94.94 | 1.16 | 95.24 | 0.95 | 79.44 | 0.81 |
| 18 | 75 (0) | 60 (0) | 0.1 (0) | 27.87 | 0.25 | 86.74 | 0.80 | 94.14 | 1.15 | 103.24 | 1.24 | 80.24 | 0.95 |
TPC, Total Phenolic Content (mg GAE/g PB); FRAP, Ferric Reducing Antioxidant Power (mmol Fe2+/g PB).