Effect of hepatic steatosis on the activities of hepatic sirtuin 1 (SIRT1), sterol regulatory element binding proteins (SREBP-1c), peroxisome proliferator-activated receptor γ coactivator-1 alpha (PGC-1α), catalase (CAT), copper-and zinc-containing superoxide dismutase (Cu/Zn SOD), manganese superoxide dismutase (Mn-SOD), glutathione peroxidase (GSH-Px) and the concentrations of glutathione (GSH), reduce glutathione disulfide (GSSG) and reactive oxygen species (ROS). Effect of lipid deposition on SIRT1, SREBP-1c and PGC-1α concentrations in control (n = 6) and fatty liver (n = 6) group dairy cows (A–C); Effect of lipid deposition on antioxidant index concentrations in control (n = 6) and fatty liver (n = 6) group dairy cows (D–H); Effect of lipid deposition on oxidative indexes concentrations in dairy cows (H–J). The data presented are the mean ± SEM. * p < 0.05; ** p < 0.01.