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. 2020 Mar 27;10(4):560. doi: 10.3390/ani10040560

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Lipid deposition enhances the hepatic lipogenesis pathway in the cow liver. (A,B) Protein levels of SREBP-1c, acyl-CoA synthetase long chain family member 1 (ACSL1), acetyl-CoA carboxylase alpha (ACCα), p-ACCα and fatty acid synthetase (FAS) in control (n = 6) and fatty liver (n = 6) group dairy cows were determined by western blotting. (C) Total RNA was extracted from liver samples and the expressions of the genes involved in free fatty acid (FFA) and triglycerides (TG) biosynthesis in control (n = 6) and fatty liver (n = 6) group dairy cows were determined by real-time RT-PCR. The data presented are the mean ± SEM. * p < 0.05; ** p < 0.01.