Fig. 3.

CD107a and cytokines release of Tan-CAR T cells responded to antigen-expressing tumor cells. (a) Degranulation of modified T cells was assessed by the appearance of lysosomal protein CD107a on the surface of the CAR T cells in response to co-culture as described. (b) Cytokine release in response to co-culture of CAR T cells and target K562 cells as indicated; cytokines evaluated include IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ, and IL-17A. Bars represent the mean ± SD of replicate samples. Data are representative of three independent experiments, each with CAR T cells generated from peripheral blood T cells from a different donor