Table 1.
Primers, annealing temperatures and product size
| Region amplified | Primer | Sequence (5′–3′) | Annealing T (°C) | Product size (bp) | |
|---|---|---|---|---|---|
| SSU HVR-I |
Forward Reverse Sequencing |
ZS6492 RS5402 ZS6492 |
AAACATGAAACCGCGGAAAG CATTCTTGGCAAATGCTTTCG AAACATGAAACCGCGGAAAG |
50 | 825 |
| SSU HVR-IV | Forward | 18SP4F | GCGAAAGCATTTGCCAA | 57 | 712 |
| Reverse | 18SPCR | ACGGCCGGTGTGTAC | |||
| Sequencing | ZS6269 | GTGGTGCATGGCCGTTC | |||
| cox1 | Forward | ZS6985 | GGTGGTTTTGGTAATTGAATG | 47 | 837 |
| Reverse | ZS6986 | ACCAGTYAAACCACCAATAGTAA | |||
| Sequencing | ZS6990 | GGTTGATAAACTATAACAGTACC | |||
Note: For more information and references see [21]
Abbreviation: T, temperature