Fig. 5.

AE and metformin inhibit high fat induced endothelial inflammation via regulating miR-146a and its target genes in HAECs. a, relative mRNA levels of NF-κB p65, IL-6, MCP-1 and ICAM-1 in HAECs transfected with miR-146a mimics. b, relative protein levels of p-NF-κB p65, IRAK1 and TRAF6 in HAECs transfected with miR-146a mimics. c, relative mRNA levels of NF-κB p65, IL-6, MCP-1 and ICAM-1 in HAECs transfected with miR-146a inhibitors. d, relative protein levels of p-NF-κB p65, IRAK1 and TRAF6 in HAECs transfected with miR-146a inhibitors. Data are presented as mean ± SEM. ^*P < 0.05 versus BSA group. ^**P < 0.01 versus BSA group. ^#P<0.05 versus control mimics or inhibitors in PA group. ^##P<0.01 versus control mimics or inhibitors in PA group. ^&P<0.05 versus control mimics or inhibitors in PA-AE group. ^ΨP<0.05 versus control mimics or inhibitors in PA-Met group. n = 5 per group for HAECs study