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Cardiomegaly and splenomegaly during the chronic stage of infection with H8 T. cruzi strain in Beagle dogs treated with DNA vaccines. The enlargement of organs was calculated by the mean heart (a) and spleen (b) indices (±S.D.). Differences were considered significant at ∗P ≤ 0.05 by the Kruskal-Wallis test among the healthy group versus the SS mock-treated, pBCSSP4, pBCSP, mixture, and pBK-CMV groups.
