Figure 7.

The effect of resveratrol on m⁶A RNA methylation in AFB₁-challenged mice. (a) qPCR analysis of m⁶A-related mRNA expression in different groups (n = 6/group). (b) Measurement of m⁶A content in different groups (n = 4/group). (c) Immunoblot analysis of m⁶A-related protein expression in different groups (n = 3/group). (d) Quantification of m⁶A-related protein expression was performed by measuring gray values using Image-Pro Plus 6.0 software (n = 3/group). CON, basal diet; RES, basal diet + 500 mg/kg resveratrol. AFB₁, basal diet + 600 μg/kg aflatoxin B₁; ARE, basal diet + resveratrol (500 mg/kg) and aflatoxin B₁ (600 μg/kg). All data were analyzed using two-way ANOVA and Duncan’s post hoc testing, where appropriate. Data are represented as mean ± SEM. ^a–c Mean values within a line with different superscript letters were significantly different (p < 0.05). (METTL3, 65–70 kDa; FTO, 58 kDa; ALKBH5, 40–50 kDa; YTHDF2, 62 kDa; β-actin, 42 kDa).