Table 4.

The beneficial effects related to physiological health observed in the chickens after a single in ovo injection with RFO prebiotic.

Days of Rearing	Day 21	Day 35	Day 42
Gut structure
Lenght of D, J, I, C, colon			+
Weight of D, J, colon			+
Weight of cecum	+
Height of the villi			+
With and surface of the villi	+
Biochemical indices
HDL			+
ALT			+
AST			+
Ca			+
Bacteriological counts
Clostridium perfringens	+		+
Oocysts counts		+	+

+ indicates a positive effect. RFO—raffinose family oligosaccharides, D—duodenum, J—jejunum, I—ileum, C—cecum, HDL—high density lipoprotein; ALT—alanine aminotransferase, AST—aspartate aminotransferase, Ca—calcium.