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Spiral ganglion, thionine staining. Following images correspond to a specific staining of the Nissl substance (arrows) for each descaling method: (a) Histofix decalcifier 3® (magnification 60×); (b) 15% formic acid (magnification 60×); (c) 10% EDTA (magnification 60×); and (d) 20% EDTA (magnification 60×).
