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Spiral ganglion, cresyl violet staining. Images show the Nissl substance (arrows) present in the spiral ganglion neurons of the ears processed with the different decalcification protocols: (a) Histofix decalcifier 3® (magnification 60×); (b) 10% EDTA (magnification 60×); and (c) 20% EDTA (magnification 60×).
