. 2020 Apr 14;10(4):683. doi: 10.3390/ani10040683

Table 2.

Performance characteristics of the different decalcifying agents used in the study. NT: not tested. HE: hematoxylin and eosin staining.

Decalcifying Agents	Decalcification Time (In Days)	Macroscopic Morphology Preservation	Microscopic Morphology Preservation		Antigenicity Conservation
Decalcifying Agents	Decalcification Time (In Days)	Macroscopic Morphology Preservation	HE	Thionine/Cresyl Violet	Antigenicity Conservation
Histofix^® Decalcifier 3	2	Very good	Good	Poor/poor	Good
15% Formic Acid	7	Poor	Good	Good/NT	NT
10% EDTA	28	Good	Poor	Fair/food	Fair
20% EDTA	25–31	Good	Very good	Very good/very good	Very good