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. 2020 Mar 31;10(4):591. doi: 10.3390/ani10040591

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Photomicrographs of the boar sperm stained with the Annexin V-FITC/PI assay kit: live sperm (AN-/PI-; blue arrow); early apoptotic sperm (AN+/PI-; white arrow); late apoptotic sperm (AN+/PI+; yellow arrow); and non-viable necrotic sperm (AN-/PI+; black arrow). Effects of different concentrations of OA (B) and PA (C) on boar sperm apoptosis. The Western blotting image shows the expression of apoptosis proteins in boar sperm after being treated with OA and PA (D). (E) Quantitative expression of the Parp-1, cleaved caspase-3, cleaved caspase-9, and p53 over α-tubulin generated from Western blotting (D). Data are the mean ± standard deviation (SD). Columns with different lowercase letters differ significantly (p < 0.05). Bars = 30 μm.