Table 2.
Chickens immunized against T-dependent (SRBC) and T-independent (dextran) antigens.
| Time | Groups and Number of Embryos/Chickens | Treatment | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| C SRBC | C DEX | Pre1 SRBC | Pre1 DEX | Pre2 SRBC | Pre2 DEX | Syn1 SRBC | Syn1 DEX | Syn2 SRBC | Syn2 DEX | ||
| D7 | 25 | 25 | 35 | 35 | 30 | 30 | 25 | 25 | 35 | 35 | 1st immunization |
| D21 | −7 | −7 | −7 | −7 | −7 | −7 | −7 | −7 | −7 | −7 | randomly selected chickens sacrificed and used for IgG, SRBC (RAT), DEX (IgM), and DEX (IgG) |
| D21 | 18 | 18 | 28 | 28 | 23 | 23 | 18 | 18 | 28 | 28 | 2nd immunization |
| D35 | −7 | −7 | −7 | −7 | −7 | −7 | −7 | −7 | −7 | −7 | randomly selected chickens sacrificed and used for IgG, SRBC (RAT), DEX (IgM), and DEX (IgG) |
C – control, Pre1 – prebiotic 1, Pre2 – prebiotic 2, Syn1 – synbiotic 1, Syn2 – synbiotic 2, SRBC – SRBC-treated groups, DEX – dextran-treated groups, IgG – serum IgG concentration, SRBC (RAT) – serum reverse agglutination titer against SRBC, DEX (IgM) – serum anti-dextran IgM antibody level, and DEX (IgG) – serum anti-dextran IgG antibody level.