Fig. 5.

Reduced percentages of Th1, Th2, Th17 cells in TOFA-treated asthma mice. IFN-γ⁺, IL-4⁺, IL-17⁺ Th cells from CD4⁺ cells, Foxp3⁺ Th cells from CD4⁺CD25⁺ Th cells in lung tissue (a) and in spleen (c) were examined by flow cytometric analysis. The percentage of CD4⁺ cells expressing IFN-γ⁺, IL-4⁺, IL-17⁺ and the percentage of CD4⁺CD25⁺ cells expressing Foxp3⁺ in lung tissue (b) and in spleen (d). All data are represented as mean ± SEM (n = 5–6 mice per group). *p < 0.05; **p < 0.01; ***p < 0.001; NS, p > 0.05