Table 4.

Regulation of perfusate

Time (min)		0–20	20	20–40	40	40–60	60	60–80	80–100	…	420
Additive	Sterile water (mL)
	Gelofusine (mL)
	Alkaline solution (mL)
	10% Calcium chloride (mL)
	Heparin (U)
	Vasoactive drugs
	Gas
	Others