Table 2.
Dynamic linear range, recovery, and matrix effects observed for AQ standards at 25 nM (LLOQ), low QC (50 nM), middle QC (250 nM), and high QC (1000 nM) concentrations. Recovery and matrix effects were calculated by comparison of peak areas from pre-extraction spike QC samples (recovery) and post-extraction spike QC samples (matrix effects) with those from QC standards prepared in neat solvent ± coefficient of variation
| Dynamic linear range | % Recovery (±%CV) | % matrix effect (±%CV) | |||||||
|---|---|---|---|---|---|---|---|---|---|
| 25 nM (LLOQ) |
50 nM (Low) |
250 nM (Med) |
1000 nM (High) |
25 nM (LLOQ) |
50 nM (Low) |
250 nM (Med) |
1000 nM (High) |
||
| C7-PQS | 25–1000 nM (R2 = 0.997) | 94.3% ± 2.4% | 105.8% ± 5.0% | 113.4% ± 6.1% | 92.0% ± 1.4% | 78.7% ± 4.7% | 83.1% ± 1.5% | 88.2% ± 3.3% | 84.7% ± 2.8% |
| C9-PQS | 25–1000 nM (R2 = 0.995) | 39.2% ± 4.0% | 55.1% ± 2.2% | 76.5% ± 5.5% | 81.8% ± 2.2% | 76.5% ± 3.6% | 82.8% ± 3.2% | 86.6% ± 3.5% | 83.2% ± 4.5% |
| HHQ | 25–1000 nM (R2 = 0.998) | 41.3% ± 2.8% | 49.3% ± 2.3% | 68.2% ± 3.9% | 81.9% ± 4.8% | 83.3% ± 2.3% | 85.1% ± 1.53% | 87.1% ± 2.9% | 86.5% ± 4.6% |
| NHQ | 25–1000 nM (R2 = 0.998) | 68.7% ± 5.7% | 80.1% ± 7.0% | 80.0% ± 2.1% | 83.8% ± 4.9% | 129.5% ± 10.5% | 129.0% ± 6.4% | 123.1% ± 5.6% | 122.1% ± 5.0% |
| HQNO | 25–1000 nM (R2 = 0.997) | 100.9% ± 4.3% | 98.8% ± 3.9% | 103.0% ± 5.6% | 89.3% ± 5.6% | 16.0% ± 1.6% | 33.6% ± 1.8% | 67.1% ± 2.3% | 78.3% ± 3.7% |
| NQNO | 25–1000 nM (R2 = 0.997) | 78.0% ± 4.0% | 85.6% ± 3.6% | 93.7% ± 4.1% | 80.2% ± 2.4% | 18.3% ± 1.7% | 40.7% ± 2.4% | 74.5% ± 4.2% | 90.2% ± 5.0% |