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. 2020 Jan 13;21(2):135–144. doi: 10.1038/s41590-019-0571-2

Extended Data Fig. 8. Loss of circadian patterns in Bmal1∆N and CXCR4∆N mice.

Extended Data Fig. 8

a, Survival of wild-type, Bmal1∆N and CXCR4∆N mice subjected to ALI at night (ZT13, solid line) or daytime (ZT5, dashed line); n = 16 mice (ZT5) and 18 mice (ZT13) for wild-type, n = 10 mice per time point for Bmal1∆N; n = 12 mice (ZT5) and 14 mice (ZT13) for CXCR4∆N. b, Representative confocal images (top) and quantification of granule content (bottom) in CXCR4-deficient neutrophils at ZT13 and ZT5. Note the loss of diurnal fluctuations compared with wild-type mice (see Fig. 1e); n = 30 cells (from 3 mice) per time point; scale, 2 μm. c, Ex vivo NET formation after PMA stimulation by CXCR4∆N neutrophils analyzed at ZT13 (n = 3 mice) and ZT5 (n = 3 mice). Note the loss of diurnal changes in NET-formation compared with wild-type cells (see Fig. 2b); d, Neutrophil counts in blood at ZT5 and ZT13 in wild-type (n = 5 mice at ZT5 and 4 mice at ZT13) and Bmal1∆N mice (n = 4 mice per time point). Data are shown as mean ± SEM. *, p < 0.05; **, p < 0.01; n.s., not significant, as determined by two-sided log rank (Mantel-Cox) test (a) or unpaired two-tailed t-test (b-d).