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A. Subcloning the FliC gene. Lanes 1-2: PCR products of FliC; lane M, DL2000 DNA Marker (Takara, Dalian, China). B. pET28a-FliC digested with BamHI and HindIII. Lane 1, pET28a-FliC; lane M, marker DL2000. C. Expression of flagellin. Lanes 1-3: SDS-PAGE analysis of flagellin. Lane 1, pET28a; lane 2, pET28a-FliC; lane 3, purified flagellin. D. Western blotting analysis of flagellin with an anti-His tag monoclonal antibody. Lane 1, pET28a; lane 2, pET28a-FliC
