Table 3.

In vitro antibacterial activity of indeno[1,2-c]pyrazol-4-ones (4a–o) through agar well diffusion method

Compounds	Zone of inhibition/mm (concentration in µg/mL)a
	Gram-positive bacteria										Gram-negative bacteria
	B. subtilis					S. aureus					E.coli					P. aeruginosa
	1000	500	250	125	Mean ± SD	1000	500	250	125	Mean ± SD	1000	500	250	125	Mean ± SD	1000	500	250	125	Mean ± SD
4a	13	12	12	11	12.00 ± 0.81	11	11	10	9	10.25 ± 0.95	10	10	10	9	09.75 ± 0.50	12	11	11	10	11.00 ± 0.81
4b	14	12	12	11	12.25 ± 1.25	12	11	11	11	11.25 ± 0.50	12	11	11	10	11.00 ± 0.81	14	13	11	11	12.25 ± 1.50
4c	12	12	11	10	11.25 ± 0.95	13	12	12	11	12.00 ± 0.81	10	9	9	9	09.25 ± 0.50	12	11	11	10	11.00 ± 0.81
4d	13	12	12	12	12.25 ± 0.50	11	10	10	10	10.25 ± 0.50	12	12	12	10	11.50 ± 1.00	11	11	11	10	10.75 ± 0.50
4e	14	13	13	12	13.00 ± 0.81	12	12	11	10	11.25 ± 0.95	11	11	11	10	10.75 ± 0.50	11	−	−	−	11.00 ± 0.00
4f	−	−	−	−	−	12	10	10	10	10.50 ± 1.00	13	12	12	11	12.00 ± 0.81	13	12	12	11	12.00 ± 0.81
4g	15	14	14	12	13.75 ± 1.25	14	13	13	12	13.00 ± 0.81	−	−	−	−	−	11	11	9	9	10.00 ± 1.15
4h	11	11	11	10	10.75 ± 0.50	11	−	−	−	11.00 ± 0.00	11	11	10	10	10.50 ± 0.57	−	−	−	−	−
4i	12	11	11	11	11.25 ± 0.50	12	11	11	−	11.33 ± 0.57	12	11	10	10	10.75 ± 0.95	11	10	10	9	10.00 ± 0.81
4j	−	−	−	−	−	12	11	11	11	11.25 ± 0.50	−	−	−	−	−	13	12	11	11	11.75 ± 0.95
4k	12	11	11	11	11.25 ± 0.50	−	−	−	−	−	15	13	12	12	13.00 ± 1.41	13	12	12	11	12.00 ± 0.81
4l	16	14	13	13	14.00 ± 1.41	14	11	11	11	11.75 ± 1.50	10	11	10	9	10.00 ± 0.81	15	13	12	11	12.75 ± 1.70
4m	13	13	12	12	12.50 ± 0.57	13	12	12	12	12.25 ± 0.50	11	11	10	9	10.25 ± 0.95	11	10	10	10	10.25 ± 0.50
4n	12	11	11	11	11.25 ± 0.50	11	11	10	10	10.50 ± 0.57	14	13	13	12	13.00 ± 0.81	−	−	−	−	−
4o	12	12	11	11	11.50 ± 0.57	21	14	11	11	14.25 ± 4.71	12	10	10	9	10.25 ± 1.25	14	11	11	11	11.75 ± 1.50
Streptomycin	26	24	22	21	23.25 ± 2.21	30	22	22	20	23.50 ± 4.43	28	24	22	18	23.00 ± 4.16	21	20	20	17	19.5 ± 1.73

^aValues including diameter of the well (8 mm) are means of three replicate