. 2019 Aug 10;28(1):169–185. doi: 10.1007/s10499-019-00451-w

Table 2.

PCR methods used to detect AHPND

Type (method)	Primers (5′-3′)	Size	Reference
One step (AP1)	AHPND Primer set 1 AP1F: CCTTGGGTGTGCTTAGAGGATG AP1R: GCAAACTATCGCGCAGAACACC	700 bp	Flegel and Lo 2013
One step (AP2)	AHPND Primer set 2 AP2F: TCACCCGAATGCTCGCTTGTGG AP2R: CGTCGCTACTGTCTAGCTGAAG	700 bp
One step (89F/R)	89F: GTCGCTACTGTCTAGCTGAA 89R: ACGGCAAGACTTAGTGTACC	470 bp	Nunan et al. (2014)
One step (TUMSAT-Vp3)	TUMSAT-Vp3 F: TGTTGCATAATTTTTGTGCA TUMSAT-Vp3 R: TGTACAGAAACCACGACTA	360 bp	Tinwongger et al. (2014)
One step (AP3)	AP3-F: ATGAGTAACAATATAAAACATGAAAC AP3-R: GTGGTAATAGATTGTACAGAA	333 bp	Sirikharin et al. (2014)
Two step (AP4)	Step 1 AP4-F1: ATGAGTAACAATATAAAACATGAAAC (= AP3-F)^a AP4-R1: ACGATTTCGACGTTCCCCAA	1269 bp	Dangtip et al. (2015)
Two step (AP4)	Step 2 AP4-F2: TTGAGAATACGGGACGTGGG AP4-R2: GTTAGTCATGTGAGCACCTTC	230 bp	Dangtip et al. (2015)
One step (duplex)	VpPirA-284F: TGACTATTCTCACGATTGGACTG VpPirA-284R: CACGACTAGCGCCATTGTTA	284 bp	Han et al. (2015c)
One step (duplex)	VpPirB-392F: TGATGAAGTGATGGGTGCTC VpPirB-392R: TGTAAGCGCCGTTTAACTCA	392 bp	Han et al. (2015c)
TaqMan qPCR	VpPirA_F: TTGGACTGTCGAACCAAACG VpPirA_R: GCACCCCATTGGTATTGAATG Probe: AGACAGCAAACATACACCTATCATCCCGCA	135 bp	Han et al. (2015c)

^aPrimer AP4-F1 from AP4 method is the same to primer AP3-F from the AP3 method