Table 3.
LAMP methods for detecting AHPND
| Type (method) | Primers (5’-3’) | Size | Reference |
|---|---|---|---|
| LAMP-A3 Assay | FIB: GTATTGAATGGTAAGCTCCCCGGAAGTCGGTCGTAGTGT | 40bp | Kongrueng et al. (2015) |
| BIP: AATGGGGTGCGCCATTTATGAAGTTTCATCACGTTGTACC | 40bp | ||
| F3: GCAAACATACACCTATCATCC | 21bp | ||
| B3: GCATTATCAGGGCGTTGT | 18bp | ||
| LF: ACGTCCCGTATTCTCAATGTCT | 22bp | ||
| LB: GCTGGCGGCTGGAAAGT | 17bp | ||
| LAMP-AuNP Assay | F3-EMS: GTGCAATTTAATAGGAGAACATC | 23bp | Arunrut et al. (2016) |
| B3-EMS: GAATGGTAAGCTCCCCAC | 18bp | ||
| FIP-EMS: CGTTTGGTTCGACAGTCCAATTTTTATGAGTAACAATATAAAACATGA | 48bp | ||
| BIP-EMS: GAGGCGTCACAGAAGTAGACATTTTCCCGTATTCTCAATGTCTACAC | 47bp | ||
| LF-EMS: CGTGAGAATAGTCAGTT | 17bp | ||
| LB-EMS: ACATACACCTATCATCCCGGAAG | 23bp | ||
| Probe-Thiol-EMS: (SH)A10-ATCATCCCGGAAGTCGGTCG | 30bp | ||
| AHPND-LAMP Assay | F3: TGATAATGCATTCTATCATCAGC | 23bp | Koiwai et al. (2016) |
| B3: ATTTGAAAGACCAAATGAAACC | 22bp | ||
| FIP: | |||
| F1c: GTGAGCACCTTCTTAGTGGTAATA | 24bp | ||
| F2: GTTGTAATTAACAATGGCGCTAG | 23bp | ||
| BIP: | |||
| B1c: TGACGGAATTTAACCCTAACAATGC | 25bp | ||
| B2: GCTTTGAAAGCATAGTTAGGATC | 23bp |