Fig. 4.

Reduction in intracellular ROS production in human leucocytes by NL13 peptide after stimulation with DCF dye as Hydrogen peroxide as free radical generator. Microscopic images (magnification = 10× and Scale bar = 100 µm) taken at different concentrations: a Control (without treatment), b NL13 at 250 µM, c NL13 at 125 µM, d NL13 at 62.3 µM, e fluorometric analysis of different concentration of peptides against ROS production. Single asterisk (*) represents P < 0.05 compared to the control by Tukey’s Multiple Comparison test in Graph Pad 5.0. The values were provided in mean of three replicates ± standard deviation