. 2019 May 23;194(1):121–134. doi: 10.1007/s12011-019-01742-2

Table 4.

Primer specification

Gene	Sequence	T_a (annealing temperature) in °C
BAX	5′ TGCTTCAGGGTTTCATCCA 3′ (Forward) 5′ GGCGGCAATCATCCTCTG 3′ (Reverse)	58
β-actin	5′TCACCACACCACTGTGCCCATCTACGA3′ (Forward) 5′AGCGGAACCGCTCATTGCCAATGG 3′ (Reverse)	60
Bcl₂	5′ AGGAAGTGAACATTTCGGTGAC 3′ (Forward) 5′ GCTCAGTTCCAGGACCAGGA 3′ (Reverse)	57

Gene

Sequence

T_a (annealing temperature) in °C

BAX

5′ TGCTTCAGGGTTTCATCCA 3′ (Forward)

5′ GGCGGCAATCATCCTCTG 3′ (Reverse)

β-actin

5′TCACCACACCACTGTGCCCATCTACGA3′ (Forward)

5′AGCGGAACCGCTCATTGCCAATGG 3′ (Reverse)

Bcl₂

5′ AGGAAGTGAACATTTCGGTGAC 3′ (Forward)

5′ GCTCAGTTCCAGGACCAGGA 3′ (Reverse)