Fig. 2.
Specific anti-CsPmy IgM levels in oral immunized grass carp Grass carp was orally administrated with different dosage of recombinant spores. Specific anti-CsPmy antibodies were determined by ELISA in week 2, 4, and 6 after the beginning of the oral immunization. (A) sera, (B) skin mucus, (C) intestinal mucus, (D) bile. Naïve, basal diet group; B.s-CotC-108: basal diet plus 1 × 108 CFU/g B.s-CotC spores. B.s-CotC-CsPmy-105, 108, 1011, basal diet plus 105, 108, 1011 CFU/g B.s-CotC-CsPmy spores, respectively. Data were represented as mean ± SD. Statistical significance was analyzed by the Dunnett’s test. *P < 0.05, **P < 0.01, ***P < 0.001