Fig. 3.
The relative mRNA levels of IgM and IgZ in the head kidney and spleen. The mRNA levels of IgM and IgZ were analyzed by qRT-PCR at 6 weeks after the beginning of the immunization. (A) IgM mRNA level in the head kidney; (B) IgZ mRNA level in the head kidney; (C) IgM mRNA level in the spleen; (D) IgZ mRNA level in the spleen. Naïve, basal diet group; B.s-CotC-108: basal diet plus 1 × 108 CFU/g B.s-CotC spores. B.s-CotC-CsPmy-105, 108, 1011, basal diet plus 105, 108, 1011 CFU/g B.s-CotC-CsPmy spores, respectively. *P < 0.05, **P < 0.01 (compared with naïve group); #P < 0.05, ## P < 0.01 (compared with B.s-CotC-108 group)