Fig. 5.

Protect effect in orally administrated fishes. Fishes were sacrificed at 4 weeks after the challenge infection. Fish flesh in each group was weighted and digested with artificial gastric juice (PH = 2.0) for metacercaria detection. (A) C. sinensis cercaria used for challenge infection. (B) Metacercariae found in grass carp after challenge infection. (C) Amount of metacercaria per gram fish flesh. Naïve, fishes fed with basal diet. B.s-CotC-10⁸, fishes fed with basal diet plus 1 × 10⁸ CFU/g B.s-CotC spores; B.s-CotC-CsPmy-10¹¹, fishes fed with basal diet plus 10¹¹ CFU/g B.s-CotC-CsPmy spores. **P < 0.01(compared with Naïve group), ##P < 0.05 (compared with B.s-CotC-10⁸ group). 100 magnification for A and 200 magnification for B