Table 2.
Primers employed in the present study
| Primer | Sequence | Binding position | Length | Purpose | Reference sequence |
|---|---|---|---|---|---|
| pPEDV-F-ZY171 | GATATGTTTGTAATGGTAACTC | 23063-23564 | 502 | Detection of PEDV (targeting the S gene) | MF807952 |
| pPEDV-R-ZY172 | AGCATAGCTAAAAGGCAATGC | ||||
| pTGEV-F-ZY173 | GATATGTTTGTAATGGTAACCC | 22946-23244 | 299 | Detection of TGEV (targeting the S gene) | KX900402 |
| pTGEV-R-ZY174 | CTCTATAGCTGAACGATACTTAC | ||||
| pPoRV-F-ZY175 | TTTACTCTACATAAAGCATCAAT | 171-586 | 416 | Detection of PORV (targeting the NSP4 gene) | KX453793 |
| pPoRV-R-ZY176 | GACGGCAACTCAACCTCTCACAT | ||||
| PEDV-ORF3-F | TCCTAGACTTCAACCTTACG | 24740-25570 | 831 | Sequencing | AF353511 |
| PEDV-ORF3-R | GGTGACAAGTGAAGCACAGA | ||||
| PEDV-S1-F | GGTAAGTTGCTAGTGCGTAA | 20570-23004 | 2435 | Sequencing | AF353511 |
| PEDV-S1-R | AATACTCATACTAAAGTTGGTGG |