Fig. 3.

a Effect of C3 deficient and reconstituted human serum on CHPV replication Vero E-6 cells were infected with “C3 deficient/reconstituted human serum/NHS7 + CHPV” (100 TCID 50). Viral RNA was extracted 24 h PI and was detected by measuring P gene RNA copies by real-time RT-PCR. “C3 reconstituted + CHPV” shows drastic inhibition in CHPV replication compared to other groups. Statistics were performed on 3 independent experiments. Values are given as mean log 10 CHPV RNA copies/well. Standard bars represent standard deviation. Statistical significance was determined by unpaired student t test. b Effect of C3 deficient and reconstituted human serum on CHPV infectivity by TC-LDA. Vero E-6 cells were infected with “C3 deficient/C3 reconstituted serum +CHPV” or “NHS7 + CHPV” (100 TCID 50, 24 h post-infection). Cells were then fixed, permeabilized, and stained with mouse polyclonal purified anti-CHPV antibody followed by the addition of anti-mouse Alexa Flour 680. C3 reconstituted serum shows a significant decrease in CHPV-positive cells percentage compared to other groups. Statistics were performed on three independent experiments. Values are given as mean ± SD of CHPV-positive cell percent of parent population. Statistical significance was determined by student unpaired t test