Table 3.

Validation for the detection of staphylococcal enterotoxins A (SEA), B (SEB) and D (SED) using ELISA^c.

Parameters	SEA	SEB	SED
Accuracy, %	7.8	12	10
Precision, %CV	5.4	2.7	7.6
limit of detection, ng/mL	0.25	0.43	0.43
limit of quantification, ng/mL	0.54	2.4	0.41
LLOQa, ng/mL	0.16	0.83	0.16
ULOQb, ng/mL	200	200	200

^aLLOQ, lower limit of quantification

^bULOQ, upper limit of quantification

^cValidation values were from supernantant human milk sample containing 25 ng/ml of SEA, SEB or SED with 4 days and 3 replicated for each day