Fig. 5.

Peli2 expression antagonizes miR-802-mediated suppression of LPS-induced proinflammatory response. a The protein expressions of Peli2 in lung tissues between LPS-induced ARDS model and sham group were compared by ELISA analysis (n = 15, for each group). The primary lung macrophages were co-transfected with miRNA or Peli2 before LPS challenge. 24 h later, the gene expression levels of b TNFα, c IL-1β and d IL-6 with or without LPS stimulation were quantified by real-time PCR. Independent experiments were repeated in triplicate. **p < 0.01, ***p < 0.001, compared with DMSO control; ^##p < 0.01, ^###p < 0.001 compared with no Peli2 control in the same treatment condition