Table 1.
DCs subsets stimulated with Brucella and their antigens
| Stimuli/dose | DCs subset | Time (stimulation/infection) | Results obtained | Reference |
|---|---|---|---|---|
| B. abortus 2308 (MOI = 300) | Human GM-DCs | 24 h | ↓CD80, CD83, CD86, IFNβ | Gorvel et al., 2014 |
|
B. suis 1330 (MOI = 5) B. abortus 2308 (MOI = 5) |
Human GM-DCs | 24 h | ↓CD40, CD83, MHC-II, ↓CD86, TNFα, IL-12 | Billard et al. 2007a, b |
|
HKBa (B. abortus 2308) (MOI = 50) L-Omp19 (10–1000 ng/mL) |
Human GM-DCs | 24 h |
↑CD80, CD86, CD83, CD40, CCR7, MHC-II ↑Il-12, IL6, IL-10, TNFα |
Zwerdling et al. 2008 |
| HKBa (B. abortus 2308) (109/mL) | Murine bmGM-Dcs | 24 h |
↑CD40, CD86, MHC-II ↑IL-12p40, TNFα |
Macedo et al. 2008 |
| B. abortus | Murine bm-GM-DCs | 24 h |
↓CD40, CD80, CD86, MHC-II ↓IL-12p70, IL-6, IFNβ, TNFα |
Salcedo et al. 2008 |
| B. abortus 2308 (MOI = 100) | Bovine moGM-DCs | 24 h |
↓CD80, CD86 ↓IL-12p40, IFNγ |
Heller et al. 2012 |
| B. abortus 2308 (MOI = 100) |
Murine bmGM-DCs GM/FL-DCs FL-DCs GM/15-DCs |
48 h 24 h |
↑CD80, CD86, MHC-II ↑IL-10 ↑IL-12, IL-6, IFNγ, IFNβ, IL-1β ↑CCL2, CCR7 |
Papadopoulos et al. 2016 |
|
OMVs B. melitensis 16 M (40 μg/mL) OMVs B. melitensis VTRM1 (40 μg/mL) |
Murine bmGM-DCs | 12 h |
↑IL-6, IL-4, IL-10 ↑IL-12, IFNγ, TNFα |
Avila-Calderón et al. 2012 |
| LPS from B. abortus 2308 ΔwadC (10 μg/mL) | Murine bmGM-DCs | 24 h |
↑CD86, MHC-II ↑IL-12, TNFα |
Conde-Álvarez et al., 2012 |
|
LPS from B. melitensis 16 M ΔwadC (20 μg/mL) LPS from B. melitensis 16 M (30 μg/mL) |
Human GM-DCs Murine FL-DCs Murine FL-DCs |
24 h |
↑TNFa, IL-12p40, IL-6, IL-10 CD80, CD40, CD86, MHC-II ↑IL-1β, IL-6, IL-12p70, IFNγ ↓CD80, CD40, MHC-II, CD86 ↓IL-10, IL-6, TNFα |
Zhao et al., 2018 |
| B. canis strain SCL (MOI = 200) |
Human GM-DCs Canine GM-DCs |
24 h |
↑CD86, CD83 ↑IL-12p35, TNFα, IL-1β ↑CD86, MHC-II ↑IL-17A, IL-6, IL-12p35, IFNγ |
Pujol et al. 2017 |
| HKBa (B. abortus 2308) (108/mL) | Splenic CD11c+ DCs (TLR9−/−) | Overnight | ↓IL-12 | Huang et al. 2005 |
| HKBa (B. abortus 119.3) (108/mL) |
Splenic CD11c+ DCs Splenic CD11c+ DCs (TLR2−/−) |
15 h |
↑IL-12 ↓IL-12 |
Zhang et al. 2012 |
|
B. abortus RB51 (MOI = 10) B. abortus 2308 (MOI = 100) |
Murine bmGM-DCs | 24 h |
↑MHC-II, CD40, CD86 ↑IL-12, TNFα ↓MHC-II, CD40, CD86 ↓IL-12, TNFα |
Surendran et al. 2012 |
| B. abortus 2308 RNA (2 μg/mL) | Murine bmGM-DCs | 24 h | ↑IL-6, IL-12, TNFα | Campos et al. 2017 |
| B. abortus 2308 (MOI = 100) |
Murine bmGM-DCs (IRAK-4−/−) |
24 h | ↓IL-12, TNFα | Oliveira et al. 2011 |
↓Downregulation
↑Upregulation