Table 1.

Primers used for amplification of selected regions of the genes encoding the HCV E2, core, NS3, and E1 proteins and the peptide P1 by PCR

Region/ endonucleases	Sense primer	Antisense primer
E2 (408-560) XbaI/HindIII/XhoI	5´-TCTAGAAGTCAGAAAATCCA -3´	5´- CTCGAGAAGCTTGAACCCAGTGCTATT-3´
Core (110-160) HindIII/XhoI	5´-AAGCTTACTGACCCCCGGCGTAG -3´	5´- CTCGAGGTCCTCCAGAACCCGGA-3´
NS3 (1230-1433) XbaI/EcoRI/XbaI	5´- GCTCTAGATGCTCCCACTGGCAGCGGC-3´	5´-CTCTAGAGAATTCAACGACGTCTCCGATAGT -3´
NS3 (1230-1341) XhoI/XhoI	5´- CTCGAGGCTCCCACTGGCAGCGG-3´	5´- CTCGAGCGGCGCTCCAGCCGTCT -3´
E1 (190-222) HindIII/EcoV/XhoI	5´- AAGCTTGATATCTCCGCCTATGAGGTG-3´	5´-AAGCTTCTCGAGCATGATCATGTCGTC-3´
P1 XhoI/XhoI	5’-TTACATCTCGAGCTGCCCGAGTACGTGATC-3’	5-TTACATCTCGAGGGCGCGGCTGGGCAGCTT-3’