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. 2020 May 14;10:7994. doi: 10.1038/s41598-020-64888-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypical HSC antigen analysis post MEISi injections. (A) Schematic showing the intraperitoneal injections of MEIS inhibitors and BM analysis at day 10. In vivo analysis of HSC compartment post MEISi treatments was carried out by analysis of (B) c-Kit⁺, (C) Sca-1⁺ cell content, (D) CD150⁺ cell content, (E) LSK cell content, (F) LSKCD34^low cell content and (G) LSKCD48⁻CD150⁺ HSC content in the whole bone marrow following injection of MEISi-1, MEISi-2 and DMSO control. (H) Expression profile of Meis1 and related target genes post MEISi injections in the whole bone marrow cells. n = 3, *p < 0.05.