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. 2020 May 14;10:7994. doi: 10.1038/s41598-020-64888-3

Table 4.

HSC gene pool primers.

Gene Forward Primer Reverse Primer
GAPDH TTGATGGCAACAATCTCCAC CGTCCCGTAGACAAAATGGT
B-Actin ATGGAGGGGAATACAGCCC TTCTTTGCAGCTCCTTCGTT
Sirt1 GACGGTATCTATGCTCGCCT ACACAGAGACGGCTGGAACT
Sipa1 CGAGGAAGCTTCTGGAACAT CAGTACAGGATGCCCACCTT
Tcp11/2 AACATAGCTCCACGCCTAGC CTGGAGAGCTGACCGACAAG
Gli1 GAGGTTGGGATGAAGAAGCA GGAGACAGCATGGCTCACTA
Als2 GAGAAGAGCGCGGAGCTG CTTTGAGTCCATCGGTCAGG
Tpm1 CTCCGAGGCTCTCAAAGATG TCCAACTCCTCCTCAACCAG
Map3k7 CTCGTCCTCCTCCTCGTCTT CAAAAGCTCCTCTTCCGACA
IL-2 AACTCCCCAGGATGCTCAC CGCAGAGGTCCAAGTTCATC
Zfp317 TGAATTTTGTTTCTTGACATTTCC GGAGACAGGGGATCCTGTTA
RB1 ACAGATTTGTCCTTCCCGTG CCATGATTCGATGCTCACAT
Txnip GATGCAAGGGTCTCAGCAGT AGCTCGAAGCCGAACTTGTA
AHR TAGGCTCAGCGTCAGCTACC CTCCTTCTTGCAAATCCTGC
Hif1a GGCGAGAACGAGAAGAAAAA GAGCTCACATTGTGGGGAAG
Dnm1l TGCCTCAGATCGTCGTAGTG CGTGGACTAGCTGCAGAATG
Inpp5d GTGAAGAACCTCATGGGGAC GCTGTTCCGGAATTGTGTTT
Cbl TGCACGGACTAAGATTCCAG ATCGCTCGCTTCGACTTCTT
Itch CCTTATGTAGAAGTCACAGTAG TCACCTACAAGCTGCAAAGTCA
Vwa9 AACCTGCTTGGAGTCTGCAT AGCTAGGGAGTGTCGCAGTG
Meis1 GTTGTCCAAGCCATCACCTT ATCCACTCGTTCAGGAGGAA
Calu GGAGCTCACTCCCTGGGT AGGCTTGCTCAAAGCAAAGG
Apc CAGCTTTTACAGTCCCAGGC TCTGACCACTACTGGAGGCTG
Cited2 GGCTGTCCCTCTATGTGCTG CATATGGTCTGCCATTTCCA
CD81 CTGCACCAAATGCATCAAAT GTACAGCAGGCTGGTGGTCT
Homer1 GGTGGAAAAGCAACTGCAA ACATGAGCTCGAGTGCTGAA
Spp1 TTGGCAGTGATTTGCTTTTG TCTGCTTCTGAGATGGGTCA
Skp2 GGCAAAGGGAGTGACAAAGA AGGAGCAGCTCATCTGGAAG
Clip4 GCAGTGTTGGGAGAGTCCA TCTCGTGGAAGGAGTGTGTG
Rbl1 AATGGTCCAGGAAACACGAC GGAAAGTACGGGGTGAGCTA
Gfi1b CCAGAAGTCGGACATGAAG TTGAAGCCTGTGTGCTTGCGG
Trp53 CTAGCATTCAGGCCCTCATC AATGTCTCCTGGCTCAGAGG
St6galnac6 GCAGCAGTTTGACGACCTCT ATCACCATGGTAAACCAGCC
C030006k11Rik GCAGGTCTTTGAAGCAGGTG CAGAACCCGCGAGTATTTCT
Dnmt3a TACATCAGCAAACGGAAACG CCTCCTCCACCTTCTGAGACT
Akt1 CATGAACGACGTAGCCATTG GCCGTTCCTTGTAGCCAATA
Rbl2 TGAAGCAATGCCTTCTCCTC TTCCGTCGTCCAAGAGAATC
Cfl2 ATGACTTCATCATTCACTGTAACTCC TCCTTCTTCTCGTCCCAGTG
Tsc-1 TGAGGTAAACAGCTGAGGGG ACTGTTCTGGGGACAGATGG
Matk TGAAGCCAAGGAGGAAACAG TGTGCTCCCAGAGTCAGATG
hTet2 AGAAAAGGGAAAGGAGAGCG GAGAGGGTGTGCTGCTGAAT
Myc TGAAGTTCACGTTGAGGGG AGAGCTCCTCGAGCTGTTTG
Elf4 AGCCCAGTGATCTGGTCTTC AGGATAGGGGACCTGTTCCA
Akt2 TGGACCACAGTCATCGAGAG CTTGTAATCCATGGCGTCCT
Eci2 CGAGTTGGCTGAATGGAGTA CCAGCTGTGGGAATCTCTGT
cdc42 GGGATCTGAAGGCTGTCAAG CTTCTTCGGTTCTGGAGGCT
Gfi1 TGGAGCTCTGACTGAAGGCT TCCCTGTCAGTACTGTGGCA
Hoxa9 ACAATGCCGAGAATGAGAGC GTTCCAGCGTCTGGTGTTTT
Runx1 TTGCCACCTACCATAGAGCC GGTGGACAGAGGAAGAGGTG
Egr1 GAGCGAACAACCCTATGAGC GGCCAGTATAGGTGATGGGA
Id1 GAGTCTGAAGTCGGGACCAC CCTCAGCGACACAAGATGC
Myb TGTCCTCAAAGCCTTTACCG GCGTTCACGTATTTCCGAG
Cdkn2c GGGGGACCTAGAGCAACTTAC CTCCGGATTTCCAAGTTTCA
Nr4a1 AGTTGGGGGAGTGTGCTAGA CTCGTTGCTGGTGTTCCATA
Ptpmt1 GTGCATTGTAAGGCTGGTCG GGCGATCGCTTCTATAGCCT
Nfat5 CGCCCAAGTCCCTGTACTC AACTCCTTTGGGAGAAGATCA
Foxo3 TTGTCCCAGATCTACGAGTGG CTGTGCAGGGACAGGTTGT