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. 2019 Apr 5;26(12):2637–2651. doi: 10.1038/s41418-019-0325-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Inappropriate activity of NDK-1 in mir-35(gk262) mutant result in increased MAPK activity and germ cell death after exposure to genotoxic stress. Number of corpses at a different irradiation dosages in N2 and mir-35-41(gk262) mutant (P < 0.001 (****), P = 0.0002 (***), n.s. = not significant) and b at 60 Gy for the indicated times (P < 0.0001 (****), P = 0.0005 (***), P = 0.0076 (**), P < 0.01 (*)). Mean fluorescence intensity (MFI) of the pachytene region was determined in isolated germlines stained for p-MPK-1 c without RNAi treatment (P < 0.0001 (****), P = 0.0025 (**), P = 0.01 (*)) or d with RNAi treatment (P < 0.0004 (***), P = 0.0012 (**), P = 0.01 (*)). Refer to Supplementary Fig 1 and 2, respectively, for representative images used in quantifying p-MPK-1. e Corpses numbers in N2 and mr-35(gk262) worms fed with the indicated RNAi and irradiated at young adult (P < 0.0001 (****), n.s. = not significant). f ced-1(e1735) on the indicated RNAi and irradiated at young adult (P = 0.0174 (*)). Corpses were determined 24 h after irradiation (n ≥ 30 worms in three independent replicates). g Germ cell corpses and h cell corpse persistence was determined in unirradiated N2, mir-35(gk262) and ced-1(e1735) worms at 24, 48 and 72 h post-L4. P < 0.0001 (****), P = 0.0007 (***). Graphs are plotted as whisker and box plot to illustrate data distribution with symbols indicating outliers based on Tukey’s test. Part (b) is a line plot with error bars indicating SEM. Part (h) is a scatter plot with errors, illustrated in red for visual ease, indicating SEM