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. 2019 Apr 5;26(12):2637–2651. doi: 10.1038/s41418-019-0325-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — miR-35 inhibits extraneous germ cell death by negatively regulating both egl-1 and ndk-1 expression. a Cell death is inhibited in mir-35(gk262); egl-1(n1084 n3082) loss-of-function double mutant (P < 0.0001 (****)). Worms were irradiated at young adult stage and corpses counted 24 h after irradiation. Number of corpses in b egl-1 3′-UTR (miR-35 mutant) (P < 0.0001 (****)) or c ndk-1 3′-UTR (mir-35 mutant) (P < 0.0001 (****), P = 0.0017 (**)) or d in the double mutant egl-1 3′-UTR (mir-35 mut.); ndk-1 3′-UTR (miR-35 mutant) young adults at 0 or 60 Gy (P < 0.0001 (****), P = 0.003 (**), n.s. = not significant). Worms were irradiated at young adult stage and corpses counted 24 h after irradiation. Symbols indicate outliers based on Tukey’s test (n ≥ 45 worms in three independent replicates)