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Validation of combinatorial library screens and the design of caspase-2 selective substrates. a The rate of hydrolysis (expressed as RFU/s, relative fluorescence units per second) of a panel of pentapeptide substrates (10 μM) with five human caspases (50 nM). b A detailed kinetic analysis of three most selective substrates for caspase-2 (top panel: chemical structures of pentapeptide substrates; lower panel: kcat/KM matrix presented as heat-map)
