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. 2020 May 14;10:7968. doi: 10.1038/s41598-020-64937-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Autofluorescence intensity and lifetime analysis. Three-dimensional representation of the skin autofluorescence (green) and second-harmonic generation (red) for healthy volunteers (a) and atopic dermatitis patients (b) with highlighted regions showing cell morphology and corresponding FLIM images (c and d, respectively). FLIM images are color-coded for the mean autofluorescence (τm) lifetime as indicated in the color bar and autofluorescence intensity weighted. Scale bar = 20 µm. Changes with depth of τm lifetime (c) and a1/a2 ratio (d) in the living cell layers of healthy and atopic dermatitis diagnosed skin. FLIM images were analyzed using the commercial software SPCImage v8.0 (Becker & Hickl GmbH, Berlin, Germany).